neda mansouri; abolfazl movafagh; mojgan shekibapour; afshin moradi; niloufar safavi; mohammad hassan heidari
Volume 1, Issue 2 , October 2021, , Pages 73-79
Abstract
Background: Abnormalities in mitosis parameter and cycle cell program are announced to be based on both the cell proliferation conditions and the condition of the metaphases of each person to respond to Phytohaemagglutinin (PHA) working condition. The aim of the current research is hence to comparison ...
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Background: Abnormalities in mitosis parameter and cycle cell program are announced to be based on both the cell proliferation conditions and the condition of the metaphases of each person to respond to Phytohaemagglutinin (PHA) working condition. The aim of the current research is hence to comparison of mitotic index outcome in the individuals of normal volunteers involved in two different period of culture with effect of PHA.Methods: Comparison of healthy human donors lymphocytes were cultured for 48-h and 72-h and using conventional karyotype technique with minor modification.Results: Mitotic index and cell proliferation frequency were analyzed in the individuals of normal blood donors after preparing of colchcine to cultured metaphases 2 hours before cell fixation at. The metaphases indics at 48-h and 72-h culture period maintained a perfect yield level. Lymphocytes populations in 72-houres laboratory work were more than 48-h cultures (2.73% at 72 hours vs. 1.48% at 48 hours, p ≤ 0.05).Conclusion: The present research indicates significant comparisons for laboratories and individuals to be set up. With effect of these results it may help in time consuming and short time period for patient, as well as fresh suggestion for health care management decision. Reagent, manual, budget, time for diagnosis, and health care fulfillment may resolve of this proposal
seyed jamal hosseini; ali nazemi; mehrdad hashemi; mirsaed miri nargesi; Sharashout sharifi; abolfazl movafagh
Volume 1, Issue 2 , October 2021, , Pages 80-85
Abstract
Introduction: High resolution melting (HRM) analysis is a technique to measure the decrease of fluorescing, caused by a discharge of the dye, throughout the DNA thermal melting gradient process. STK11 gene encodes one of the cellular serine-threonine kinase proteins that regulate cellular polarity. It ...
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Introduction: High resolution melting (HRM) analysis is a technique to measure the decrease of fluorescing, caused by a discharge of the dye, throughout the DNA thermal melting gradient process. STK11 gene encodes one of the cellular serine-threonine kinase proteins that regulate cellular polarity. It also acts as a tumor suppressor protein. Mutations of the germline in the gene are coincident with Peutz-Jeghers syndrome and the potential of developing a variety of neoplasia.Method: In an experimental study, the genome DNA of 56 patients with digestive system cancer was extracted. Afterward, nucleotide changes over the gene were examined using Real-time PCR and High resolution melting (HRM).Findings: Nucleotide screening using the HRM technique revealed two types of SNP in introns 6 & 7 in 10 patients. Four patients showed homozygous C/T nucleotide changes [cluster id/dsSNP/rs9282860] in intron 6 and six patients showed heterozygous C/G nucleotide changes [cluster id/dsSNP/rs2075607] in intron 7. A comparison of HRM results with sequencing results indicated 100% conformity.Conclusion: Although no mutation was observed in exon section of the gene, primary screening of the STK11 gene to diagnose unknown nucleotide changes of germline and somatic in patients with neoplasia using HRM was feasible, easy, and cost-effective.
fatemeh Shaabanpour Aghamaleki; mojgan Sheikhpour; sima Kianpour Rad; iman Ekramnia; aliasghar Keramatinia; narjes mehrvar; niloufar Safavi Naini; abolfazl Movafagh
Volume 1, Issue 1 , July 2021, , Pages 29-34
Abstract
Transposable elements are mobile genetic units that exhibit broad diversity in their structure and transposition mechanisms. Transposable elements occupy a large fraction of many eukaryotic nucleic acid, protein and their movement and accumulation represent a major force shaping the genes and genomes ...
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Transposable elements are mobile genetic units that exhibit broad diversity in their structure and transposition mechanisms. Transposable elements occupy a large fraction of many eukaryotic nucleic acid, protein and their movement and accumulation represent a major force shaping the genes and genomes of almost all organisms. Sequence repeats are an important phenomenon in the human genome, playing important roles in genomic alteration often with phenotypic consequences. The two major types of repeat elements in the human genome are tandem repeats (TRs) including microsatellites, minisatellites, and satellites and transposable elements (TEs).Conclusion: TEs are contributing to genome expansion and alteration not only by transposition but also by generating tandem repeats. Finally, this article highlight how the distinctive biological features of DNA transposons have contributed to shape genome architecture and led to the emergence of genetic innovations in different eukaryotic nucleic acid, protein lineages.
